Loss of alloreactivity after 4 repetitive in vitro stimulations with CMV antigen.

After 4 repetitive stimulations with CMV antigen, a complete loss of alloreactivity (black bars) was documented in the CMV-specific T-cell line generated from the patient's haploidentical (3 Ag-mismatched) father (patient no. 2). In addition, no autoreactivity (gray bars) was detected prior to and after stimulation with CMV antigen.