Fig. 3.
![Fig. 3. Dose- and time-dependent inhibition of inflammatory cytokines by ritonavir. / Human PBMCs were stimulated with anti-CD3 mAbs and treated with the indicated concentrations of ritonavir (30 μmol/L [♦], 10 μmol/L [▴], 3 μmol/L [▪], and untreated control [○]). Supernatants were collected at indicated time points, cleared, and assayed for cytokines by ELISA, (A) IFN-γ, (B) TNF-α, (C) IL-1β, and (D) IL-6. Results from one representative experiment are shown of 4 performed with similar data. Data points illustrated represent means of duplicate determinations, SD ≤ 12%.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/10/10.1182_blood.v99.10.3771/6/h81022573003.jpeg?Expires=1724859377&Signature=zucz3drlp6ygWqIxlT-8AVqASkF0YxVirkeQvxDNuWK13-YIbI7u9Z9HpghgLOuz~B8vcCjUPXhdW2XVkUkOFY3YvtpnX1XY60gu8yKeahjeJJa~pVH7qKZGbNwN0h86y14C6d88~QlQF~gb18F2pa5DV0BWx5dSS4e-ijiyvrJmIWD6G6vfHWakcM3W9WBOfdX4kC-~-t31I-zrsUfG0KJEfi1~65zapbbnOounqx9JrrwDxVvaEh2NPZ0kEsA7d~ZGU9D6RT1rTxQGZ~CJHmPuflpEHus6a58moEszmbvvapwlK65DFpBafVcFfSA0562oBSg6ZttdFiVJxzlgeg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dose- and time-dependent inhibition of inflammatory cytokines by ritonavir.
Human PBMCs were stimulated with anti-CD3 mAbs and treated with the indicated concentrations of ritonavir (30 μmol/L [♦], 10 μmol/L [▴], 3 μmol/L [▪], and untreated control [○]). Supernatants were collected at indicated time points, cleared, and assayed for cytokines by ELISA, (A) IFN-γ, (B) TNF-α, (C) IL-1β, and (D) IL-6. Results from one representative experiment are shown of 4 performed with similar data. Data points illustrated represent means of duplicate determinations, SD ≤ 12%.
