Fig. 3.
Adhesion of HSPCs to adherent layer of LTBMCs is CD44 variant independent.
Enriched HSPCs were incubated in 4 wells at a concentration of 103 cells/well with irradiated adherent cells of LTBMCs for 4 hours with CD44-specific antibodies (10 μg/mL) as indicated. After washing to remove unbound cells and monoclonal antibodies, bound cells were further cultured for 14 days, and the numbers of cobblestone areas were counted and presented as means ± SD. Con indicates control antibody (10 μg/mL); pan, IM7 antibody (10 μg/mL). The experiment was repeated 3 times with similar results.
