Induction of erythroid differentiation in Friend 707 cells by DMSO decreases frataxin protein levels.

Western blot analysis was performed using proteins extracted from cells incubated in the presence (+) or absence (−) of 1.5% DMSO for 24 to 120 hours at 37°C. The membrane was sequentially probed with antifrataxin and anti-β-actin antibodies (see “Materials and methods” for details). Densitometric analysis of the results normalized to the β-actin loading control are shown below the blot. The result illustrated is a typical experiment from 4 experiments performed.