Inhibition of collagen-bound VWF by 82D6A3.

Different concentrations of 82D6A3 were either preincubated with diluted human plasma (final dilution of 1:46, ie, 217 ng/mL VWF) before addition to a collagen-coated plate (●) or were added to a collagen-coated plate where VWF (final dilution of 1:46, ie, 217 ng/mL VWF) was already bound for 30 minutes (○). Bound VWF was detected with rabbit antihuman VWF antibodies.