Fig. 4.
β-Catenin contributes to homotypic cell aggregation of PHA-activated Jurkat cells.
Jurkat cells were transiently transfected with vectors encoding pEGFP (5 μg) and other constructs (10 μg each) as indicated, and GFP-positive cells were sorted by flow cytometry, suspended in complete medium containing 2 μg/mL PHA, and plated into a 96-well plate at 5 × 104/100 μL per well. After 30 minutes of incubation at 37°C, the cells were transferred to a hemocytometer using a wide-diameter pipette tip, and aggregation was determined using an inverted Leica microscope. Single cells, small clusters containing 2 to 10 cells, and clusters of more than 10 cells were counted by visual inspection of at least 4 wells per condition in 3 independent experiments.
