Experimental AIHA in CD47^−/− and wild-type (CD47^+/+) C57BL/6J mice.

Experimental AIHA was induced in CD47^−/− (open symbols) or wild-type (closed symbols) C57BL/6J mice by using (A) the antimouse RBC mAb 34-3C (mouse IgG2a), or (B) the antimouse RBC mAb 105-2H (mouse IgG1). The indicated amount of mAb 34-3C or 105-2H per gram body weight was given as an intraperitoneal injection on day 0. The mice were then followed daily for weight and hematocrit for 7 days after injection. CD47^−/− mice that received 1 μg mAb 34-3C/g body weight (panel A, open circles) succumbed at day 3 to 4 of the experiment. Data are mean ± SD for 3 to 4 mice in each group.