Sequence comparison of human VAMP isoforms.

Platelet VAMPs were isolated by coimmunoprecipitation with antisyntaxin 4 antibodies. The sequences of VAMP-3 and VAMP-8 identified by microcapillary reverse-phase HPLC–nano-electrospray tandem MS are shown in boldface type. Alignments were carried out by using the ClustalW service at the European Bioinformatics Institute (http://www.ebi.ac.uk/clustalw). The 260-amino acid VAMP-7 is not shown in the alignment. Portions of VAMP-4 (N-terminal 25 amino acids, MPPKFKRHLNDDDVTGSVKSERRNL) and VAMP-5 (amino acids 95-116, QSSDSSSAPRTQDAGIASGPGN) also are not shown.