Expression of ILT2/CD85j on fresh circulating SS cells.

(A) Freshly isolated PBMC from 2 representative SS patients, Dc and Ao—both with a TCRVβ8⁺ circulating malignant clone—were analyzed by 2-color immunofluorescence and flow cytometry with an ILT2/CD85j-specific mAb (GHI/75), followed by FITC-conjugated anti-IgG + IgM GAM. After extensive washing to remove the excess of GAM and blocking with normal mouse Ig, the PE-conjugated anti-TCRVβ8 mAb was added. (B) Lymphocytes isolated from involved skin of a MF patient, Bic, were analyzed by 2-color immunofluorescence and flow cytometry, as described above, with the ILT2/CD85j-specific mAb and a TRI-conjugated anti-CD4 mAb. No anti-ILT2 mAb reactivity with the CD4⁺ cell subset was detected with lymphocytes isolated from the skin biopsies of another MF patient and from 2 patients with pagetoid reticulosis.