Fig. 1.
Fig. 1. Detection of ILT2 on CTCL cell lines. / The long-term CTCL cell lines Pno and Cou-L were analyzed by 2-color immunofluorescence and flow cytometry with an ILT2/CD85j-specific mAb (GHI/75), followed by FITC-conjugated anti-IgG + IgM GAM. After extensive washing to remove the excess of GAM and blocking with normal mouse Ig, the PE-conjugated anti-TCRVβ22 or anti-TCRVβ13 was added.

Detection of ILT2 on CTCL cell lines.

The long-term CTCL cell lines Pno and Cou-L were analyzed by 2-color immunofluorescence and flow cytometry with an ILT2/CD85j-specific mAb (GHI/75), followed by FITC-conjugated anti-IgG + IgM GAM. After extensive washing to remove the excess of GAM and blocking with normal mouse Ig, the PE-conjugated anti-TCRVβ22 or anti-TCRVβ13 was added.

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