TNF stimulation increases IL-21 receptor expression in OH-2 cells.

Cells were stimulated with TNF (10 ng/mL) or IL-6 (1 ng/mL) for 18 hours before the addition of streptavidin–PE-conjugated antibodies against IL-21R. Mean fluorescence intensities were 6.3 (control), 8.4 (no cytokine), 8.2 (IL-6), and 10.9 (TNF). Similar patterns were obtained in the cell line ANBL-6, and data shown are representative of 3 different experiments.