Nucleotides induce intracellular Ca⁺⁺ transients in immature and mature DCs.

Immature (A,C,E,G,I) and mature (B,D,F,H,J) DCs were loaded with fura-2/am as reported in “Materials and methods” and stimulated with nucleotides at the indicated concentrations. MIP-3β was used as a positive control for mature DCs. Data from 1 representative experiment of 5 similar are shown.