ASO-PCR monitoring and corresponding sensitivity tests.

For each detected Thr315Ile, Phe311Leu, and Met351Thr mutation, specific ASO-PCR products on ethidium-bromide–stained agarose gel are shown. (C): PCR reaction using Thr315Ile wild-type, Phe311Leu, and Met351Thr mutated primers. (T): PCR reaction using Thr315Ile mutated, Phe311Leu, and Met351Thr wild-type primers. (M): duration of STI571 therapy expressed monthly from diagnosis (M0; ie, before treatment). For sensitivity, corresponding dilution range cell numbers are indicated. Controls: ASO-PCR products on DNA obtained from healthy donor.