Fig. 1.
![Fig. 1. Staining intensity of different leukocyte populations with OX1 and anti-RT7a mAb. / LEW.1W peripheral leukocytes (obtained from whole EDTA blood samples, erythrocytes lysed by NH4Cl) were double-stained with specific antibodies (R73/T cells, OX33 B cells, 3.2.3/NK cells, and HIS48/granulocytes; indirect staining with phycoerythrin-conjugated goat anti-mouse mAb (GAM) and OX1 or anti-RT7a mAb (RT7.1 fluorescein isothiocyanate–[FITC] or OX1bio plus FITC-conjugated streptavidin, direct staining), respectively. FL1/FL2 dot plots were gated for subpopulations of total leukocytes. Representative histograms for gated cells are shown for OX1 and anti-RT7a mAb staining intensities, and mean channels of fluorescence are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/10/10.1182_blood.v99.10.3566/6/h81022544001.jpeg?Expires=1725461309&Signature=0soydj9S4WY5u3kxTMmPefD4B1fAFuYWG2g6akUCnQ7io3nUt57lNmDEAEOy83MR-nFdwAjgR9j0v9C8Q4KGE7P-C5XRQJIRSmgZLxV99pmiK8DutXjqgXto-cDHcmlRkP0cbX6sPg~vKfT6Ws7gRx~pwVwGd5zx6Rw3Yop5SynAA87QSok1-gnlDvHtZr~W29ZdwatYrdg1DICus8~xZYt7K81d~mfuzc3XDU85bz60Rb3FEJwav5kbrNzclkiwp0ZbhN4TDDyP0PBSomLxA5QhtdKwR0aotdVnqwzP4BdwCHPLRHhXyVx~QilFYRiQf0yMlfybBAERE5XTCTNY2Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Staining intensity of different leukocyte populations with OX1 and anti-RT7a mAb.
LEW.1W peripheral leukocytes (obtained from whole EDTA blood samples, erythrocytes lysed by NH4Cl) were double-stained with specific antibodies (R73/T cells, OX33 B cells, 3.2.3/NK cells, and HIS48/granulocytes; indirect staining with phycoerythrin-conjugated goat anti-mouse mAb (GAM) and OX1 or anti-RT7a mAb (RT7.1 fluorescein isothiocyanate–[FITC] or OX1bio plus FITC-conjugated streptavidin, direct staining), respectively. FL1/FL2 dot plots were gated for subpopulations of total leukocytes. Representative histograms for gated cells are shown for OX1 and anti-RT7a mAb staining intensities, and mean channels of fluorescence are indicated.
