Stability of pseudotyped SIV-vector virions in human and macaque sera.

Infectious pseudotyped SIV-vector particles (50 000 GFP IU in 50 μL suspension buffer) were mixed with 50 μL fresh (diagonal bars) or heat-inactivated (solid bars) human (A) or macaque (B) sera. As a reference, virions were mixed with 50 μL heat-inactivated FCS. Virion-sera mixtures were incubated at 37°C for 1 hour and then used to transduce TE671 target cells. Values show the titers of primate sera-incubated virions relative to the titers of the same virions incubated in FCS (%). The results of experiments performed with sera of 3 different individual donors are shown. The experiments with human serum no. 659 were performed in triplicate and are displayed as mean values ± SD.