Fig. 4.
Apoptosis analysis of Ba/F3-derived transfectants in the absence of IL-3.
Parental Ba/F3 cells and other transfectants were washed extensively and resuspended at a density of 2 × 105 to 3 × 105 cells per milliliter in the media without cytokine. The aliquots of the cells were harvested after every 24 hours, stained with propidium iodide, and analyzed by fluorescence-activated cell sorter. Each column represents the independent Ba/F3 clones of each category.
