Characterization of Ba/F3-derived transfectants.

Cell extracts prepared from the indicated cell lines were analyzed by Western blot for the tyrosine phosphorylation status and the expression of either p160 v-Abl, Akt, p110 subunit of PI 3-K, or v-H-Ras. The closed and open triangles indicate the endogenous c-Akt and introduced v-Akt, respectively.