Immunoblot analysis of intact cells directly solubilized in boiling SDS sample buffer.

Blots were probed with antiankyrin and antiprotein 4.1 antibodies. Lane 1, uninfected and ring-infected RBCs. Lane 2, trophozoite- and schizont-infected RBCs. Truncated ankyrin and protein 4.1 of approximately 155 kd and approximately 56 kd, respectively, were detected in trophozoite- and schizont-infected RBCs but not in uninfected or ring-infected RBCs. Note that in the left panel, overexposure of lane 1 did not detect the 155-kd band in uninfected and ring-infected RBCs (lane 1′).