Flow cytometry of CD19⁺ cells in spleens of vaccinated mice.

(A) Flow cytometric analysis of spleen cells from mice vaccinated with huCD20-KLH, msCD20-KLH, or P190-KLH control conjugate stained with rat antimouse CD19-FITC antibody. The X-axis is CD19 fluorescence. The Y-axis is the number of cells. (B) Percentage of CD19⁺cells in each group of vaccinated mice calculated from the data obtained in A. Bar represents the mean value ± SD. Values from individual mice are represented by circles. P values are for P190-KLH versus huCD20-KLH, and P190-KLH versus msCD20-KLH. This experiment was done twice with similar results.