Western blot analysis of CHO cell lysates.

Lysates were subjected to 8% SDS-PAGE under nonreduced conditions (A) or 10% SDS-PAGE under reduced conditions (B,C). Blots were developed with an antibody to fibrinogen (A,B) as described in the legend to Figure 2, or with a polyclonal antibody reacting with the fibrinogen Bβ and γ chains (C), as described in “Materials and methods.” Cross-reacting bands were detected by peroxidase-catalyzed chemiluminescence (A,B) or by alkaline phosphatase–catalyzed color development (C). The samples were as described in Figure 2 except the plasma fibrinogen (lanes Fbg) was 30 ng (C). Arrows labeled γ411, γ379, and γ386 indicate normal and truncated γ chains. The 47-kd band indicated with asterisk (B) was present in AαBβ-CHO cells.