Fig. 5.
Differentiation, gene induction, PML-RARα degradation, and histone acetylation by different combinations of As2O3, tRA, and HMBA or NaB in R4 cells.
R4 cells were treated with 10−6 M tRA, 0.5 μM As2O3, and 2 mM HMBA or 0.5 mM NaB, alone or as in indicated combinations, for 3 days. (A) Differentiation. Differentiation was determined by NBT reduction. (B) Gene induction. Bfl-1/A1, C/EBPε and myeloperoxidase (MPO) were determined by Northern blot analysis. (C) PML-RARα degradation and histone acetylation. PML-RARα, AcH3, and AcH4 protein levels were detected by Western blot analysis.
