Fig. 1.
Fig. 1. Collagen induces platelet O2− production. / (A) Effects of various stimuli on platelet superoxide (O2−) production. Collagen (6 μg/mL, n = 24) and phorbol-12-myristate 13-acetate (PMA, 1 μM, n = 21), but not ADP (5-50 μM, n = 9) or thrombin (0.1-1 U/mL, n = 12), increased O2− formation by platelets (10 minutes after stimulation). O2− was measured by L-012 chemiluminescence. (B) Influence on NAD(P)H oxidase and PKC inhibitors on collagen-induced (6 μg/mL) O2− formation. Kinetics of collagen-induced O2− production and its inhibition by DPI (100 μM, n = 6) and chelerythrine (50 μM, n = 10). Data are shown as mean ± SEM; * (#), ** (##) significantly different at P < .05, .01 vs stimulus and DPI (*) or chelerythrine (#).

Collagen induces platelet O2− production.

(A) Effects of various stimuli on platelet superoxide (O2−⁠) production. Collagen (6 μg/mL, n = 24) and phorbol-12-myristate 13-acetate (PMA, 1 μM, n = 21), but not ADP (5-50 μM, n = 9) or thrombin (0.1-1 U/mL, n = 12), increased O2− formation by platelets (10 minutes after stimulation). O2− was measured by L-012 chemiluminescence. (B) Influence on NAD(P)H oxidase and PKC inhibitors on collagen-induced (6 μg/mL) O2− formation. Kinetics of collagen-induced O2− production and its inhibition by DPI (100 μM, n = 6) and chelerythrine (50 μM, n = 10). Data are shown as mean ± SEM; * (#), ** (##) significantly different at P < .05, .01 vs stimulus and DPI (*) or chelerythrine (#).

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