Fig. 3.
Cell cycle analysis of transduced cells.
Immunohistochemistry on spleen (A,B), liver (C), and gut (D) sections for assessing BrdU incorporation from adult SCID (A,C,D) and Balb/c (B) mice injected intravenously with 109 TU HIV-GFP and implanted with BrdU-osmotic pumps. The analysis was performed 1 week after transduction, and green fluorescence corresponds to GFP-expressing cells. The red cells are BrdU+ cells that have proliferated, and the arrow defines lentivirally transduced nonparenchymal cells that had divided and stained BrdU+(cells that are not expressing GFP but have divided are red; cells that are expressing GFP but are not dividing are green; and cells that are expressing GFP and have divided are yellow). Scale bar is depicted.
