Fig. 8.
![Fig. 8. Immunoprecipitation of RhD and RhAG from K562 and K562/RhD/RhAG cells. / TPA-treated (+) and untreated (−) cells were incubated for 3 hours with [35S]-methionine. Cell lysates from K562 (W.T.) and K562/RhD/RhAG cells were used for immunoprecipitation with the MPC8 anti-Rh polyclonal antibody (PAb) (A) or the LA18.18 anti-RhAG mMAb (B) and Protein A–Sepharose. The isolated proteins were separated by 10% SDS-PAGE under reducing conditions. Low molecular weight proteins from Biorad were used as standards. Arrows indicate the 32-kd Rh and RhAG proteins.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/3/10.1182_blood.v100.3.1038/4/h81522885008.jpeg?Expires=1725049160&Signature=xxQutyq6KZVoyI33UPul2cbQ4c51ePRuHB9yMNPQkXWGhKaF7P1ZmtoZj9a~~uN7H21KJgCvRoV5W5~sjAf89D6V7xxbS63cfQjtRNWPHTGjA-RyQV~YTAs4o5d~3wdhdfsvpDmpK5yviFpKs09D1iyZz4OmhUog~23Vf5a85yGGSFN7Q1XI6mlGMsBKEhI5odLsrIg93V-V-zTt1KbfS-j-6wAT~zMdxPKsN2zpzKiA0luerH7Pi5w5lKJxYy~d0U-QnuVKQGcyG7P11h9E3lMwBebr7ze-Jn5ZiuCxfe3Gr~S3cXYPDkAckJR~XZueaJKkv-vkwI42-LEquAfh9A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of RhD and RhAG from K562 and K562/RhD/RhAG cells.
TPA-treated (+) and untreated (−) cells were incubated for 3 hours with [35S]-methionine. Cell lysates from K562 (W.T.) and K562/RhD/RhAG cells were used for immunoprecipitation with the MPC8 anti-Rh polyclonal antibody (PAb) (A) or the LA18.18 anti-RhAG mMAb (B) and Protein A–Sepharose. The isolated proteins were separated by 10% SDS-PAGE under reducing conditions. Low molecular weight proteins from Biorad were used as standards. Arrows indicate the 32-kd Rh and RhAG proteins.
