Flow cytometric analysis of K562 cells transiently transfected with pcDNA3, pcDNA3/RhAG, and pcDNA3/K2 vectors.

Following double immunostaining experiments (as described in “Materials and methods”), simultaneous analysis of the RhD and RhAG antigen expressions (A,B) and of RhD and K2 antigen expressions (C,D) were performed on TPA-untreated cells (left panels) and TPA-treated cells (right panels).