Flow cytometric analysis of HEK293 cells expressing recombinant RhAG and RhD antigen.

(A) The mouse MAb LA18.18 was used to compare RhAG antigen expression on hygromycin-resistant HEK293/RhAG cells (^____) and untransfected HEK293 cells (….). (B) The human MAb anti-D F5 was used to compare RhD antigen expression on hygromycin- and G418-resistant HEK293/RhAG/RhD cells (^____) and HEK293/RhD cells (….). In dot-plot representations, the R2 region was defined as a positive region in which no signal was detected with negative controls (mouse IgG1 and human anti-K1 MAb).