CD23a is differently regulated in B-CLL cells compared with normal B lymphocytes.

In vitro expression kinetic studies demonstrating that in B-CLL cells CD23a levels remain high during 5 days of incubation under serum-free conditions, whereas in normal B-cellsCD23a is down-regulated within 24 hours. (A-C) FACS analysis showing the percentage of CD23, bcl-2, and early and late apoptotic cells (Annexin V⁺ and Annexin V⁺/propidium iodide⁺, respectively). (D) RT-PCR analysis correlating the expression of Notch2 with CD23a andbcl-2.