Fig. 5.
Fig. 5. Notch2IC induces endogenous. / CD23a in B cells. The CD23−pre–B-cell line REH was transiently transfected with pSG5-rN2IC, coding for the intracellular domain of rat Notch2. After 48 hours of incubation, total RNA was subjected to RT-PCR analysis that demonstrated that rNotch2IC induces CD23a transcription (lane 3). Lane 2 shows a control transfection experiment with pCMV-GFP. B-CLL cells (lane 4) served as positive control for CD23aexpression.

Notch2IC induces endogenous

CD23a in B cells. The CD23−pre–B-cell line REH was transiently transfected with pSG5-rN2IC, coding for the intracellular domain of rat Notch2. After 48 hours of incubation, total RNA was subjected to RT-PCR analysis that demonstrated that rNotch2IC induces CD23a transcription (lane 3). Lane 2 shows a control transfection experiment with pCMV-GFP. B-CLL cells (lane 4) served as positive control for CD23aexpression.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal