Notch2 is overexpressed in B-CLL cells.

(A) Relative position of the Notch2 sequence (Notch2-RT-PCR) used for mRNA detection. The intracellular domain of Notch is characterized by the RAM23 domain and 6 copies of a cdc10/ankyrin motif, known to bind CBF1 (TM, transmembrane domain; NLS, nuclear localization signals; TAD, transactivation domain; PEST, protein degradation signal). (B) RT-PCR analysis indicating that Notch2 is overexpressed in B-CLL samples where it correlates with the expression of CD23a(PBMCs, Th cells, and peripheral blood B-cells from healthy donors are representative for n = 3). β-Actin was used as an internal control.