SDS-PAGE and IEF demonstrate an abnormal chain in fibrinogen Hillsborough.

(A) SDS-PAGE of normal (N) and propositus (P) fibrinogens under reduced conditions. The normal Aα, Bβ, and γ chains are labeled on the left and the abnormal γ chain is indicated on the right (arrow). (B) IEF of purified γ chains over the pH range of 4 to 8.