T-cell–induced damage to CVE monolayers.

CVEs were cultured 18 to 20 hours alone (A, D), with PLP139-151–specific Th1 cells and no antigen (B, E), or with 0.02% Triton X-100 (C, F). (A-C) Representative pictures were taken using a Nikon N90 AF 35-mm camera and scanned into Adobe Photoshop using Polaroid Insight for analysis (original magnification, 200×). (D-F) Monolayers were then washed and stained with DAPI (blue) and PI (red) to quantitate the total number of cells and the number of dead cells, respectively. Slides were examined and images were acquired through epifluorescence using the SPOT RT camera, and they were analyzed with Metamorph Imaging Software.