![Fig. 4. Comparison of the antigen-presenting capabilities of cytokine-treated SJL/J astrocytes and CVEs for Th1 and Th2 cells. / SJL/J CVEs were incubated for 48 hours with no cytokine, IFN-γ (100 U/mL), TNF-α (500 U/mL), or both cytokines. Cells were washed, harvested, and irradiated (30 Gy) before the addition of 5 × 104 PLP139-151–specific Th1 cells (A), Th2 cells (B), or T-cell hybridomas (C) along with antigen. Irradiated syngeneic splenocytes (4 × 105/well) were used as a control APC population in separate wells. (A, B) T-cell proliferative responses were measured by 3[H]-thymidine incorporation. Results are expressed as mean cpm (× 10−3) ± SEM of triplicate wells using 104 CVEs per well and the indicated concentration of PLP139-151. Stimulation indices are noted beside each bar. (C) T-cell hybridoma responses were measured by collecting culture supernatants after 24 hours and determining IL-2 levels by ELISA. Data are expressed as average absorbance of triplicate wells at 450 nm ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/10/10.1182_blood-2001-12-0229/6/h81022557004.jpeg?Expires=1724269522&Signature=ZTv0UvVw2cBt6GXSvtVl3Zf1RB5eOed-cMs-Y9cxlijwinhsnj6D0uGyelupU-9IzMX3b16uncoytbYPng-MirGazygOUtAkh3CZ~LvY04KJgibQP37QfarisUJ1QYeyd-bAmpOMN~RR~f2sPYeb-s47z2-B04WavKNHSD9viNShzohEVP146tem4~zcFH8GhgueV1pHjiKoQd7~ASg0F9PnSR5AEgnPeT8ii2dnlrcMoean~Njvwf6kLEAdMOUHHqw9VG~nNFU8PD0OP-emPidNWxoRBGXqVrB-pshionOvvuVFSwoOFv0~j~Aha4bncT1jPVpId3f9aK~WCftD7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Comparison of the antigen-presenting capabilities of cytokine-treated SJL/J astrocytes and CVEs for Th1 and Th2 cells.
SJL/J CVEs were incubated for 48 hours with no cytokine, IFN-γ (100 U/mL), TNF-α (500 U/mL), or both cytokines. Cells were washed, harvested, and irradiated (30 Gy) before the addition of 5 × 104 PLP139-151–specific Th1 cells (A), Th2 cells (B), or T-cell hybridomas (C) along with antigen. Irradiated syngeneic splenocytes (4 × 105/well) were used as a control APC population in separate wells. (A, B) T-cell proliferative responses were measured by 3[H]-thymidine incorporation. Results are expressed as mean cpm (× 10−3) ± SEM of triplicate wells using 104 CVEs per well and the indicated concentration of PLP139-151. Stimulation indices are noted beside each bar. (C) T-cell hybridoma responses were measured by collecting culture supernatants after 24 hours and determining IL-2 levels by ELISA. Data are expressed as average absorbance of triplicate wells at 450 nm ± SD.
