Comparison of MHC class II-restricted T-cell responses elicited by cytokine-activated SJL/J astrocytes and CVEs.

SJL/J astrocytes or SJL/J CVEs were incubated for 48 hours with no cytokine, IFN-γ (100 U/mL), TNF-α (500 U/mL), or both cytokines. (A) Cells were washed, harvested, irradiated (30 Gy), and plated (10⁴/well) before the addition of 5 × 10⁴Thy 1.2⁺ allogeneic (A/J) T cells. (B) Cells were washed, harvested, irradiated (30 Gy), and plated (10⁴/well) before the addition of 5 × 10⁴ Thy 1.2⁺ syngeneic (SJL/J) T cells and 10μg/mL SEB. Irradiated syngeneic splenocytes (4 × 10⁵/well) were used as a control APC population in separate wells in both experiments. T-cell proliferative responses were measured by ³[H]-thymidine incorporation. Results are expressed as mean cpm (× 10⁻³) ± SEM of triplicate wells. Stimulation indices are noted beside each bar.