Comparison of expression of MHC class II (A) and CIITA (B) on cytokine-treated SJL/J CVEs and astrocytes.

Cloned SJL/J CVEs or astrocytes were cultured with no cytokine, 100 U/mL IFN-γ, 500 U/mL TNF-α, or both cytokines for 48 hours. Cells were analyzed for relative expression of I-A^s by (A) flow cytometry and (B) CIITA mRNA by RT-PCR. (A) Histogram plots of the flow cytometric staining are shown plotted as the number of positive cells versus fluorescence intensity with I-A^s staining in bold. Isotype control staining is plotted in the fine line. (B) RNA was isolated from CVE and astrocyte cultures, and PCR samples were prepared as described in “Materials and methods.” Data shown are scanned images of ethidium bromide-stained 2% agarose gels.