Effect of ferric citrate on c-myc expression by human HSFC.

HSFCs were cultured in media with 1 mM ferric citrate (lane 2) or 1 mM sodium citrate (lane 3) for 7 days. The cells were harvested, total RNA was extracted, and RT-PCR was performed. The amplified samples were resolved on 1.5% agarose gels. Lane 1, DNA markers and lane 4, HSFC control cells grown in standard medium.