Fig. 3.
Effect of C2-ceramide on ferric citrate–stimulated HSFC proliferation.
HSFCs were cultured with 1 mM ferric citrate for 7 days to induce proliferation then transferred into a 96-well plate and incubated for an additional 9 days with media containing ferric citrate to which 25 or 50 μM of C2-ceramide was added and cell proliferation compared to ferric citrate alone. Cell number was determined as described for Figure 1.
