Physical interaction of wild-type and mutant PU.1 peptides with AML1, GATA-1, and c-Jun.

GST-fusion proteins for AML1 (runt domain), GATA-1, or c-Jun were incubated with in vitro translated PU.1 wild-type or one of the PU.1 mutant peptides. The interaction with GATA-1 served as a positive control, since GATA-1 interacts with both the amino and carboxyl terminus of PU.1.39 43 Input: 1/10: 10% of in vitro translated protein used for binding reaction was loaded as a control.