Fig. 3.
![Fig. 3. HPK1 enhances JNK and, to a lesser extent, ERK and p38 MAP kinase activation in H2O2-treated EL-4 cells. / EL-4 cell lines expressing wt HPK1 (pHA-HPK1 wt), a kinase-inactive K46M mutant (pHA-HPK1 M[46]), AS-HPK1 (pHA-HPK1 AS), or control vector RNA (pEGZ-HA) were treated with 1 mmol/L H2O2 for 0 to 120 minutes. 100 μg cellular proteins were resolved by SDS-PAGE, blotted, and detected with the indicated Abs. Representative blots from 4 independent experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/3/10.1182_blood-2002-01-0089/5/h81522889003.jpeg?Expires=1726867962&Signature=wDe1hbf1sWLBv8etK5oGgWUe6JHS2vujSiKPW0FJcxZUJ6ZB7Q8WhehL-k560n-jGqkRKtg6JsRLn6AMmFxmWMnmB8b-ELwprvR3vQIIw2xW1D2R5GRKJijpAvdG7dglcb3B~wzuSSdWXCkOtO-7FYkaL23sJmA7LE89gIlfhe-CfWRPHa1Dmn3TXy8IOcOtxk69LfCqzHIeZoXhZvxWtbWYdJPrbloRGua5kbMBDqToH5jqP0cggrj1RP3X5HhgnbQIg265hQbcjn6KSPASEjFjAuwwHoh6zb7ovYBjzNJI~6Z5GiH9R1i4-lGbTMg3EZqwq9lSDxEl52CdqviePQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HPK1 enhances JNK and, to a lesser extent, ERK and p38 MAP kinase activation in H2O2-treated EL-4 cells.
EL-4 cell lines expressing wt HPK1 (pHA-HPK1 wt), a kinase-inactive K46M mutant (pHA-HPK1 M[46]), AS-HPK1 (pHA-HPK1 AS), or control vector RNA (pEGZ-HA) were treated with 1 mmol/L H2O2 for 0 to 120 minutes. 100 μg cellular proteins were resolved by SDS-PAGE, blotted, and detected with the indicated Abs. Representative blots from 4 independent experiments are shown.
