Exposure to the CB2-selective agonist JWH-015 leads to reduced cell viability and induction of apoptosis in Jurkat and Molt-4 tumor cells in vitro.

(A) The effect of JWH-015 on tumor-cell viability was determined by culturing Jurkat and Molt-4 tumor cells for 24 hours in serum-free medium in the presence of various concentrations of JWH-015 (1, 5, 10, and 20 μM) or the vehicle. The viable cell number was determined by trypan blue dye exclusion. (B) The effect of JWH-015 on the induction of apoptosis in Jurkat and Molt-4 tumor cells was determined by culturing the tumor cells for 24 hours in serum-free medium in the presence of 5 μM JWH-015 (filled histogram) or the vehicle (empty histogram). Apoptosis was quantified using the TUNEL method, and the cells were analyzed using a flow cytometer.