THC and HU-210 exposure leads to the induction of apoptosis in human lymphoid tumors in vitro.

Human tumors Molt-4, Jurkat, and Sup-T1 were cultured in serum-free medium in the presence or absence of various concentrations of THC, HU-210 (2.5, 5, and 10 μM), or the vehicle for 4 hours. (A) The induction of apoptosis was determined by the TUNEL method, and the percentage of apoptotic cells was plotted. (B) A representative experiment in which human tumor cells cultured with 10 μM of THC or HU-210 (filled histograms) or the vehicle (open histograms) were analyzed for apoptosis using TUNEL assay.