C/EBPα does not recruit TSA-sensitive corepressors in down-regulating PU.1 transcriptional activity.

Transient transfection in F9 cells with a reporter construct of a minimal TK promoter with PU.1-inding sites and expression plasmids of PU.1 and C/EBPα. p(C/EBP)2TK is a control reporter for C/EBPα. TSA was used at a concentration of 100 M. Control experiments for TSA consisted of expression plasmid Gal4-Tel and a minimal promoter with GAL4 DNA-binding sites only (pGal4-luc). Luciferase activities were measured 24 hours after transfection.