Coimmunoprecipitation of PU.1 and C/EBPα proteins.

(A) Coimmunoprecipitation of PU.1 and C/EBPα from whole-cell lysates of transfected 293T cells with anti-PU.1 antibody or normal IgG. Western blot analysis was performed by using anti-C/EBPα antibody. (B) Coimmunoprecipitation of PU.1 and C/EBPα from whole-cell lysates of transfected 293T cells with anti-PU.1 antibody or normal IgG. Western blot analysis was performed by using anti-PU.1 antibody. (C) Western blot analysis of the whole-cell lysates of the cells transfected with C/EBPα, C/EBPαΔLZ, and C/EBPαmBR was performed using an anti-C/EBPα antibody.