Colocalization of PU.1 and C/EBPα in U937 cells.

U937 cells were incubated with mouse anti-PU.1 antibody and rabbit anti-C/EBPα antibody. Cy3-conjugated (green) anti-rabbit IgG and Texas red anti-mouse IgG were used as secondary antibodies. Colocalization is demonstrated by the yellow signal, generated by the overlay of the red and green signals. (Original magnification × 100).