Cytotoxicity assay with target cells of various cell lineages.

GPI⁻ cells (●) of 3 different cell lineages were less susceptible than control cells (GPI⁺, ○) to both peripheral and cultured NK cells. (A) Cytolysis of myeloid cells (K562) by cultured NK cells (2E3). (B) Cytolysis of B-lymphoid cells (JY-5) by peripheral NK cells of a healthy donor. (C) Cytolysis of T-lymphoid cells (MOLT-4) by cultured NK cells (2E3). Each value represents the mean (± SD) of triplicate assays. Each abscissa indicates E/T ratios.