CD8α⁺CD11c⁻Lin⁻ cells fail to differentiate into T cells, NK cells, or other myeloid lineage cells.

At day 21, the splenocytes from the CD8α⁺CD11c⁻Lin⁻cell–reconstituted mice were collected and stained with FITC-conjugated anti-Ly5.2 and PE-conjugated anti-CD3ε, anti-B220, anti-NK1.1, or anti–Gr-1, respectively. The quads were set up on the isotype-matched control dot plot. The results are representative of 6 independent experiments.