Allogenic MLR.

Allogenic MLR was performed by using purified CD4⁺ T cells (3 × 10⁵ cells/well in 96-round-well plate) as responder cells. ⋄ represents Ly5.2⁺CD8α⁺Ia⁺ DCs from naive Ly5.2 B6 mice (wild-type CD8α⁺ DCs), whereas ■ represents Ly5.2⁺CD8α⁺Ia⁺ DCs from Ly5.2⁺CD8α⁺CD11c⁻Lin⁻precursor-reconstituted Ly5.1 B6 mice (donor-derived CD8α⁺ DCs). ○ represents mature DCs generated from BM-derived HPCs (BM-derived DCs). All the indicated cell subpopulations were purified or generated as described in “Materials and methods.” Results are expressed as the mean ± 1 SD of the triplicate cultures and are representative of 3 independent experiments.