Isolation of CD8α⁺CD11c⁻Lin⁻ cells from BM and lymph nodes.

BM cells (A) and lymph node cells (combined axillary, cervical, inguinal, and mesenteric lymph nodes; B) were enriched by CD8α-microbeads and then stained with biotin-conjugated anti-CD11c, revealed with APC-streptavidin, as well as FITC-conjugated anti-CD8α, PE-conjugated anti-Lin markers (CD3ε, B220, Gr-1, CD11b, and NK1.1). The CD8α versus Lin markers staining was gated on CD11c⁻cells. The gate indicated the BM-derived (A) and lymph node–derived (B) CD8α⁺CD11c⁻Lin⁻ cells, respectively. The results are representative of 3 independent experiments.