Fig. 2.
The 19.ek.Fc microspheres exhibit specific adhesion to CHO-P in vitro.
The 2-μm 19.ek.Fc or ek.Fc microspheres were drawn over CHO-Ps or CHOs (negative control). In certain cases the substrate or the microspheres were pretreated with mAbs. After a set amount of time, the number of microspheres adherent to the cellular monolayers was determined. Substrate indicates CHO-P or CHO cell monolayer; ligand, which molecule was on the microsphere; mAb, pretreatment of the microsphere or substrate with the indicated mAb. KPL-1 is an antihuman PSGL-1 mAb used on the microspheres. HPDG2/3 is an antihuman P-selectin mAb used on the CHO-P cells; shear stress = 1 dyne/cm2, n ≥ 3; *P < .05; all of the adherent microspheres were firmly adherent.
