Fig. 3.
Migration of endothelial cells into a wounded area.
A standardized wound of 27 mm2 was produced in monolayers of cultured endothelial cells. The total number of single cells that migrated into the wound area at 6 hours was then counted. VE-cadherin−/− cells migrated more efficiently than VE-cadherin+/+ cells. BV13 or BV14 mAbs (50 μg/mL) were added to VE-cadherin+/+ cells immediately after the wound. Both mAbs increased cell migration to a level comparable to VE-cadherin−/− cells. Data are means ± SEM of quadruplicates from 1 experiment representative of 3 performed.
