Demonstration of the A81402T (Asp816Val) mutation and C81517T (81517C>T) polymorphism of c-kit in 187 individual mast cells by single-cell PCR amplification of genomic DNA followed by sequencing.

The regions containing the mutation and the polymorphism in the same electropherogram from 4 individual bone marrow mast cells (A-D) are shown. Panel A is typical of 12 of 187 cells in which the mutation but not the polymorphism was detected. Panels B and C are typical of 149 of 187 cells in which both the polymorphism and the mutation were detected. Panel D is typical of 26 cells in which the polymorphism but not the mutation was detected.